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Cavostelium apophysatum

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C. apophysatum on bottom right
Domain:Eukaryota
Kingdom:Amoebozoa
Phylum:Evosea
Class:Variosea
Order:Cavosteliida
Family:Cavosteliaceae
Genus:Cavostelium

Introduction

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Found in 1960 by Lindsay Olive while searching for slime molds, Cavostelium was initially classified as Protostelium. This proved to be false due to the formation of flagella the amoeboid cells developed. The inability to place Cavostelium into a distinct group resulted in a new genus and family being formed.[1]

Morphology

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Typically have a single nucleus and or multiple contractile vacuoles. Amoebae are elliptical to fan-shaped to highly branched with an angular outline. Very flat with long acutely pointed pseudopodia. Amoebae usually develop at least one flagella (usually two) and at most four (rare).[2] Microcysts are usually present and developing abundantly in spherical or irregular shapes. The irregular shapes are more frequent in crowded areas. There are also sporangia which typically have one or two spores that are normally spherical. C. apophysatum have a yellow pigment and masses of the cell in culture give off the yellow color (light orange has also been observed). Nothing is known of the nature of the pigment.[1]

Behavior

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C. apophysatum grows well and sporulates in various vultures with lactose-yeast extract agar and hay infusion agar being the best. When placed in the dark, C. apophysatum grows rapidly but does not sporulate. During periods of uncoordinated cell movement, the flagella move about in an irregular movement, while the cell continually changes its shape. The cells are poor swimmers regardless of the number of flagella present.[1]

Life Cycle

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C. apophysatum has a simple life cycle with a trophic phase consisting of flagellate cells and uninucleate amoebae. In the beginning stages of fruiting, each amoeba withdraws their pseudopodia and becomes spherical. After this shaping takes place, a protective sheath forms over the sporogen. The sporogen then forms a stalk and a spore wall.[3] Sexual processes haven’t been demonstrated in C. apophysatum.[1]

References

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  1. ^ a b c d Olive, Lindsay S. (November 1964), "A new member of the Mycetozoa", Mycologia, 56 (6): 885–896, doi:10.1080/00275514.1964.12018179
  2. ^ Spiegel, Frederick W., and Joyce Feldman. “Obligate amoebae of the protostelids: Significance for the concept of eumycetozoa.” Biosystems, vol. 18, no. 3–4, Jan. 1985, pp. 377–386, https://doi.org/10.1016/0303-2647(85)90037-1.[2]
  3. ^ Whitney, Kenneth D. “Sporocarp Ultrastructure and Development in the Protostelid Cavostelium apophysatum1.” Journal of Eukaryotic Microbiology 31 (1984): 253-259.[2]