Galactoside acetyltransferase
(Redirected from Beta-galactoside transacetylase)
| Galactoside O-acetyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 GAT in complex with CoA and two molecules/active site of IPTG viewed perpendicular to the molecular threefold axis of the enzyme | |||||||||
| Identifiers | |||||||||
| EC no. | 2.3.1.18 | ||||||||
| CAS no. | 9029-94-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
| Thiogalactoside acetyltransferase | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | lacA | ||||||
| Entrez | 945674 | ||||||
| RefSeq (Prot) | NP_414876 | ||||||
| UniProt | P07464 | ||||||
| |||||||
Galactoside acetyltransferase (also known as Galactoside O-acetyltransferase, thiogalactoside transacetylase, β-galactoside transacetylase and GAT) is an enzyme that transfers an acetyl group from acetyl-CoA to β-galactosides, glucosides and lactosides. It is coded for by the lacA gene of the lac operon in E. coli.[1]
Reaction
[edit]It catalyzes the following reaction:
acetyl-CoA + beta-D-galactoside → CoA + 6-acetyl-beta-D-galactoside
The kinetics of the enzyme were delineated in 1995.[2]
Biological role
[edit]The enzyme's role in the classical E. coli lac operon remains unclear.[1][3] However, the enzyme's cellular role may be to detoxify non-metabolizable pyranosides by acetylating them and preventing their reentry into the cell.[1][4]
See also
[edit]References
[edit]- ^ a b c Wang, Xing-Guo; Olsen, Laurence R; Roderick, Steven L (April 2002). "Structure of the lac Operon Galactoside Acetyltransferase". Structure. 10 (4): 581–588. doi:10.1016/S0969-2126(02)00741-4. PMID 11937062.
- ^ Lewendon, Ann; Ellis, Jacqueline; Shaw, William V. (November 1995). "Structural and Mechanistic Studies of Galactoside Acetyltransferase, the Escherichia coli LacA Gene Product". Journal of Biological Chemistry. 270 (44): 26326–26331. doi:10.1074/jbc.270.44.26326. PMID 7592843.
- ^ Roderick, Steven L. (26 May 2005). "The lac operon galactoside acetyltransferase". Comptes Rendus. Biologies. 328 (6): 568–575. doi:10.1016/j.crvi.2005.03.005.
- ^ Andrews, Kenneth J.; Lin, E.C. (October 1976). "Thiogalactoside transacetylase of the lactose operon as an enzyme for detoxification". Journal of Bacteriology. 128 (1): 510–513. doi:10.1128/jb.128.1.510-513.1976. PMC 232886.
External links
[edit]
Media related to Galactoside acetyltransferase at Wikimedia Commons- galactoside+acetyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
- EC 2.3.1.18
