The 2000 Ukrainian Amateur Cup was the fifth annual season of Ukraine's football knockout competition for amateur football teams. The competition started on 2 July 2000 and concluded on 15 October 2000.
Twelve regions did not provide any teams for the tournament, among which were Crimea, the oblasts of Dnipropetrovsk, Ivano-Frankivsk, Kherson, Mykolaiv, Odesa, Poltava, Rivne, Sumy, Ternopil, Vinnytsia, and Zaporizhia.
The following is the bracket that demonstrates the last four rounds of the Ukrainian Cup, including the final match. Numbers in parentheses next to the match score represent the results of a penalty shoot-out.